mouse brain endothelial cell line bend3  (ATCC)

Journal: Frontiers in Immunology

Article Title: Serum EMMPRIN/CD147 promotes the lung pre-metastatic niche in a D2A1 mammary carcinoma mouse model

doi: 10.3389/fimmu.2025.1568578

Figure Lengend Snippet: EMMPRIN promotes angiogenesis in the lung PMN. Mice were injected with the different tumor cells and treatments as described in the legend of Figure 1 . (A) Representative images of the endothelial cell marker CD31 staining in the lungs, and (C) their quantitation (n=9 for the D2A1-WT group, n=8 for the D2A1-KD group, n=7 for the healthy group, n=7 for the D2A1-WT + m161-pAb group, n=9 for the Healthy + rec. EMMRPIN group). Bar size is 100 μm. (B) Representative images of bEND3 endothelial cell migration length after their incubation for 18 h with serum-starvation media containing 5µg of lung lysates from the different experimental groups. (D) Quantitation of the migration length (n=14 for the D2A1-WT group, n=10 for the D2A1-KD group, n=8 for the healthy group, n=9 for the D2A1-WT + m161-pAb group, n=7 for the Healthy + rec. EMMRPIN group). Bar size is 150 μm. Data are presented as mean ± SEM. Three groups were analyzed using one-way ANOVA followed by Bonferroni’s post-hoc test, and two groups were compared using the non-parametric two-tailed Mann-Whitney t test. The increased CD31 staining of endothelial cells in the mice implanted with the D2A1-WT cells and their longer migration distance suggests that they proliferate and migrate, two properties necessary for angiogenesis, more than the endothelial cells in healthy mice or mice implanted with the D2A1-KD cells. The importance of EMMPRIN to this process is exemplified by the results from the addition of h161-pAb or the injection of recombinant EMMPRIN.

Article Snippet: The mouse brain endothelial cell line bEND3 (ATCC CRL-2299) was cultured in the same full medium that contained 1% glutamine, whereas D2A1 full medium contained 2% glutamine.

Techniques: Injection, Marker, Staining, Quantitation Assay, Migration, Incubation, Two Tailed Test, MANN-WHITNEY, Recombinant

Journal: Antioxidants

Article Title: Plant Extract of Limonium gmelinii Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion

doi: 10.3390/antiox10111814

Figure Lengend Snippet: L. gmelinii extract reduces ROS in human primary astrocytes ( a ) and bEnd3 cells ( b ) treated with TNF-α and H 2 O 2 . The level of ROS is represented by the relative intensity of CM-H2DCFDA normalized to the control group. ***— p ≤ 0.001 compared to the control; **— p ≤ 0.01 compared to the control; ●— p ≤ 0.05 compared to the group treated with TNF-α; ○○○— p ≤ 0.001 compared to the group treated with H 2 O 2 .

Article Snippet: We have chosen mouse bEnd3 cerebral endothelial cell line (ATCC) as a model of human brain cerebral endothelial cells since we have previously reported that this cell line responds to the different treatments in a similar way to human primary CECs [ ].

Techniques: Control

Journal: Antioxidants

Article Title: Plant Extract of Limonium gmelinii Attenuates Oxidative Responses in Neurons, Astrocytes, and Cerebral Endothelial Cells In Vitro and Improves Motor Functions of Rats after Middle Cerebral Artery Occlusion

doi: 10.3390/antiox10111814

Figure Lengend Snippet: L. gmelinii extract suppresses TNF-α and H 2 O 2 -induced expression of P-selectin on the cell surface. Fluorescent images of P-selectin labeled bEnd3cells ( a ); relative intensity of P-selectin in the bEnd3 ( b ). ***— p ≤ 0.001 compared to the control ; *— p ≤ 0.05 compared to the control ; •— p ≤ 0.05 compared to the cells treated only with TNF-α; ○○○—compared to the cells treated only with H 2 O 2 .

Article Snippet: We have chosen mouse bEnd3 cerebral endothelial cell line (ATCC) as a model of human brain cerebral endothelial cells since we have previously reported that this cell line responds to the different treatments in a similar way to human primary CECs [ ].

Techniques: Expressing, Labeling, Control